Diode laser in delayed replantation: histometric study in rat teeth
145644 Diode laser in delayed replantation: histometric study in rat teeth
Friday, March 18, 2011: 3:30 p.m. - 4:45 p.m.
Location: Hall C (San Diego Convention Center)
Presentation Type: Poster Session
Presentation Type: Poster Session
Objectives: The aim of this study was to evaluate by histometric analysis, the effects of high power diode laser irradiation in root surfaces of delayed replanted rat teeth. Methods: Sixty rat maxillary right incisors (Rattus norvegicus, albinus, Wistar) were extracted and were allowed to bench-dry for sixty minutes. After, the the pulp was extirpated by a retrograde way, and the root canal was prepared and filled with calcium hydroxide paste (Calen®). According to the root surface treatment before the replantation, the teeth were assigned to four groups (n=15): G1 (negative control) – no root surface treatment; G2 (positive control)- treated with 2% sodium fluoride solution; G3- irradiated with high power diode laser(810nm), continuous mode, optic fiber 600 µm, 1.5 W input, 30s) and G4 - irradiated with high power diode laser in the same parameters of G3, but in pulsed mode. The rats were killed by anesthetic overdose 15, 30 and 60 days after replantation and the specimens were processed for histometric analysis. It was calculated the mean (%) of the root resorption areas for each group. Results: In all experimental periods (15, 30 and 60 days), the root resorption percentage mean was in decreasing order: G1>G2>G4>G3.The diode laser irradiated groups (G3 and G4) presented the least means and there was no statistical difference (P< 0.05) between them. Replacement resorption and ankylosis were observed only at periods of 30 and 60 days, but in G3 such events were not observed. Conclusions: The root surface treatment with high power diode laser irradiation before delayed replantation, in the parameters used in this study, reduced external root resorption prevalence. In continuous mode, it inhibited replacement resorption and ankylosis emergence.
Keywords: Endodontics, Lasers, Reimplantation and Resorption
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